Dr. Anju Pappachan
Assistant Professor
Department of Bioinformatics & Structural Biology
School of Biological Sciences and Biotechnology
Indian Institute of Advanced Research
Koba, Gandhinagar, Gujarat, India
Ph: +91-79-
30514152
Fax: +91-79-30514110
E-mail:
anju@iiar.res.in 

Academics:

PhD: 2009 from Molecular Biophysics Unit, Indian Institute of Science,Bangalore

M.Sc: 2003 from Tamil Nadu Agricultural University, Coimbatore.


Research Areas:

Leishmaniasis is a spectrum of diseases caused by the protozoan parasite Leishmania sp.Visceral leishmaniasis caused by Leishmania donovaniis the most fatal. High toxicity and cost of current treatments and the emergence of drug resistant parasites point to an urgent need for the development of new classes of drugs against Leishmania. In this context, novel drug targets have to be identified.A rational approach to develop new drugs is to identify metabolic differences between the host and parasite and exploit those proteins which are crucial for the survival of the parasite, at the same time not significant for the host as drug targets.


Our group is involved in characterizing proteins belonging to the purine salvage pathway of
Leishmania donovani. Many of these proteins are potential antileishmanial drug targets. Structural and functional characterization of these proteins will be carried out using protein engineering, X-ray crystallography, biophysical techniques, enzyme functional assays and bioinformatic tools. The group is also involved in similar study of kinases involved in carbon metabolism, proteins belong to trypanothione metabolism and methyl glyoxal detoxification pathways of L.donovani. Insights from these studies will open up avenues for the development of new anti Leishmanials.


Publications
:

 

  1. Patel D. K., Shah K. R. , Pappachan A., Gupta S., Singh D. D. (2016) Cloning, expression and characterization of a mucin-binding GAPDH from Lactobacillus acidophilus. Int J Biol Macromol. http://dx.doi.org/10.1016/j.ijbiomac.2016.04.041
  2. Shah KR, Patel DK, Pappachan A, Prabha CR, Singh DD.  (2015) Characterization of a Kunitz-type serine protease inhibitor from Solanum tuberosum having lectin activity. Int J Biol Macromol. 83;259-69.
  3. Singh V., Nair D., Kaushal R. S., Kumar M., Pappachan A., Singh D. D. (2015) Heterologous expression, purification and characterization of L-type lectin homologue from Leishmania donovani. Biotechnology Reports, 8: 81-87. doi:10.1016/j.btre.2015.09.004
  4. Yamuna Kalyani M, Pappachan A,Savithri HS, Murthy MR. Dramatic structural changes resulting from the loss of a crucial hydrogen-bond in the hinge region involved in C-Terminal helix swapping in SurE: A survival protein from Salmonella typhimurium. PLoS One. 2013,8(2):e55978
  5. Xavier JR, Gnanam R, Murugan MP, Pappachan A. Clonal propagation of Phyllanthus amarus: A hepatoprotector. Pharmacogn Mag. 2012. Jan 8(29):78-82.
  6. Pappachan A, Chinnathambi S, Sathesh kumar PS, Savithri HS, Murthy MR. A single point mutation disrupts the capsid assembly in Sesbania Mosaic Virus resulting in a stable isolated dimer. Virology. 2009. Sep 30: 392(2)-215-21.
  7. Pappachan A, Savithri HS, Murthy MR. Structural and functional studies on a mesophilic stationary phase survival protein (Sur E) from Salmonella typhimurium.  FEBS J. 2008 Dec;275(23):5855-64.
  8. Pappachan A, Subashchandrabose C, Satheshkumar PS, Savithri HS, Murthy MR. Structure of recombinant capsids formed by the beta-annulus deletion mutant - rCP (Delta48-59) of Sesbania mosaic virus. Virology. 2008 May 25;375(1):190-6. 
  9. Pappachan A,Gnanam R, Ambika KS. Micro propagation in Phyllanthus amarus.Schum. And Thonn. Advanced biotech.in 2007 April.

 

 

Conference Presentation:

  • Patel. B, Patel D, Chauhan R, Singh DD, Pappachan A. “Xanthine phosphoribosyl transferase (XPRT) – A potential anti-leishmanial drug target”. International Conference on Biomolecules forms & functions, January 8-11, 2013, Indian Institute of Science, Bangalore
  • Pappachan A, Subashchandrabose C, Satheshkumar PS, Savithri HS, Murthy MR. “Structural studies on Sesbania mosaic virus capsid protein”. EMBO World Lecture series on Recent Developments in Macromolecular Crystallography, November 9-15, 2008, National Chemical Laboratory, Pune.
  • Pappachan A, Subashchandrabose C, Satheshkumar PS, Savithri HS, Murthy MR. “Structural studies on the β-annulus deletion mutant of Sesbania mosaic virus coat protein (CP-∆48-59)” . In-house symposium, June, 2007, Indian Institute of Science, Bangalore.
  • Pappachan A, Subashchandrabose C, Satheshkumar PS, Savithri HS, Murthy MR. “Mutational studies on interfacial residues of Sesbania mosaic virus coat protein”. International Symposium on Recent Trends in Macromolecular Structure and Function, January 18–20, 2006, University of Madras, Chennai.
  • Pappachan A, Gnanam R. “Engineering the carotenoid biosynthetic pathway”.  National Level Conference on Frontiers in Enzyme Technology, November 15- 16, 2002, Karpagam Arts and Science College, Coimbatore.