Dr. Rajesh Singh

Dr. Rajesh Singh
Cell Biology Group
School of Biological Sciences and Biotechnology
Indian Institute of Advanced Research
Koba, Gandhinagar, Gujarat, India
Ph: +91-79-30514240
Fax: +91-79-30514110
E-mail: rsinghiiar.res.in
 

Research Interests
Identification of new cell death regulators

Introduction: Aim: Identification of new Regulators of Apoptosis: 

Programmed cell death is conserved phenomenon from lower eukaryotes to human. Basic well orchestrated program of cell death, also known as apoptosis, plays important role from development to adult organisms to maintain normal cellular homeostasis. This is especially critical in mammals that integrate multiple physiological as well as pathological death signals. The process of apoptosis is initiated in response to infectious agents, different type of stresses ranging from envorimental to cellular stress. Any gain- and loss-of-function of the core apoptotic pathway can be a primary pathogenic event that results in disease. Evidence indicates that insufficient apoptosis can manifest as cancer or autoimmunity, while accelerated cell death is evident in acute and chronic degenerative diseases, immunodeficiency, and infertility. Although cell death was observed from long time however its active field of research witnessed an explosion of information over the last decade. It is now well established that there are two basic mechanisms of apoptosis. One that senses the extracellular signals of cell death known as extrinsic pathway and other intrinsic pathway of apoptosis mediated through mitochondrial signaling.

After the spurt in the field to identify the new regulators using classical biochemical approaches, we have still not been able to identify the targets in the diseased state e.g. in cancer, neurodegeneration, autoimmunity, diabetes and etc. In the postgenomic era, where we know the possible postulated genes in the human genome, there is need to use the high throughput techniques to identify the new regulators of apoptosis. We are planning to use high throughput siRNA approach to identify new regulators of apoptosis and also during inflammation and innate immunity. Newly identified molecules will be targeted for drug therapy by designing specific inhibitor/activator using bioinformatics and combinatorial chemistry in collaboration with other groups in the institutes. With the advent of posttranscriptional regulation of signaling pathways through micro RNA (miRNA), it is important to identify if apoptosis is also regulated at this levels also.

 

 

Research Group:

 

Awards and Fellowship

1.Marie Curie More Experienced Researcher NUI, Galway-2007-08, Ireland
2.Selected for Indo-US-STF Fellowship award-2011-2012
3.Session Chair at “International Conference on Recent Advances in Cancer Research: Bench to Bedside”. February 19-20, 2011, at School of Life Sciences, Central University of Gujarat, Gandhinagar


Seminars/Workshops/Conferences Organized

A symposium on "Emerging Trends in Cell Biology" from April 27-28, 2010

 

Representative Publications

1. Rajesh Singh, Geeta Upadhyay, Amit Kapoor, Sanjeev Kumar, Ashok Kumar, Meenakshi Tiwari and Madan M. Godbole (2002) Hypothyroidism alters expression of Bcl-2 family genes to induce apoptosis in rat developing cerebellum. J Endocrinology 176(1): 39-46.
 
2. Rajesh Singh, Geeta Upadhyay and Madan M. Godbole (2002) Hypothyroidism alters mitochondrial morphology to induce release of apoptogenic proteins in developing rat cerebellum. J Endocrinology 176(3):321-329.
 
3. Geeta Upadhyay, Rajesh Singh, Gaurav Agarwal, Lily Pal, Pradahn PK, Das BK, Saroj K. Misra and Madan M. Godbole (2002) Enhanced Expression of Sodium Iodide Symporter (hNIS) Gene in human breast carcinoma tissue. Breast Can Res Treatment 77:157-165.
 
4. Geeta Upadhyay, Rajesh Singh, Ramesh Sharma, Anil K Balapure, Madan M. Godbole (2002) Iodine acts directly on mitochondria from tumoral breast tissue to induce apoptosis independent of cytochrome c. Mitochondrion 2(3):199-210.
 
5. Geeta Upadhyay, Rajesh Singh, Amit Kapoor, Sanjeev Kumar, Ashok Kumar, Menakshi Tiwari and Madan M. Godbole (2004) Severe hyperthyroidism induces mitochondrial-mediated apoptosis in rat liver. Hepatalogy 39(4):1120-30.
 
6. Singh R*, Gupta S, Datta P, Zhang Z, Orr C, Lu Z, DuBois G, Zervos AS, Meisler MH, Srinivasula SM, Fernandes-Alnemri T, Alnemri ES (2004) The carboxy terminal tail of presenilin regulates Omi/HtrA2 protease activity. J Biol Chem. Oct 29; 279(44): 45844-54.
 
7. Kumar A, Sinha RA, Tiwari M, Pal L, Shrivastava A, Singh R, Kumar K, Kumar Gupta S, Godbole MM. Increased pro-Nerve Growth Factor and p75NTR levels in developing hypothyroid rat cerebral cortex are associated with enhanced apoptosis. Endocrinology. 2006 Jun 22; (Epub).

 

8. Tomar D, Singh R, Singh AK, Pandya CD, Singh R. TRIM13 regulates ER stress induced autophagy and clonogenic ability of the cells. Biochim Biophys Acta. Mol Cell Res. 2012 Feb;1823(2):316-26. Epub 2011 Dec 8.

 

9. Singh AK, Singh R, Tomar D, Pandya CD, Singh R. The leucine aminopeptidase of Staphylococcus aureus is secreted and contributes to biofilm formation. Int J Infect Dis. 2012 May;16(5):e375-81. Epub 2012 Mar 10.

 

10. Sripada L, Tomar D, Prajapati P, Singh R, Singh AK, Singh R. Systematic Analysis of Small RNAs Associated with Human Mitochondria by Deep Sequencing: Detailed Analysis of Mitochondrial Associated miRNA. PLoS One. 2012;7(9):e44873.  Epub 2012 Sep 11.

 

11. Tomar D, Sripada L, Prajapati P, Singh R, Singh AK, Singh R (2012b) Nucleo-cytoplasmic trafficking of TRIM8, a novel oncogene, is involved in positive regulation of TNF induced NF-κB pathway. PloS One. Article in press.

 

12. Sripada L, Tomar D, Singh R (2012b) Mitochondria: One of the destinations of miRNAs. Mitochondrion. Article in press.

 

* Contributed equally

 



Abstracts and/or proceedings:


1. Tomar D, Singh AK, Singh R. Conjugation of endogenous Escherichia coli and Staphylococcus aureus proteins through human small ubiquitin like modifier (SUMO) protein.  International Conference on Emerging Trends in Biotechnology, Banaras Hindu University, Varanasi, India,  December 04-06, 2009.

2. Singh AK, Singh R, Tomar D, Pandya C, Singh R. Identification  and biochemical characterization of Leucyl aminopeptidase from  Staphylococcus aureus. International Conference on Understanding and Managing Pathogenic Microbes, Institute of Microbial Technology, Chandigarh, India, January 22-24, 2010.

3. Tomar D, Singh R, Pandya C, Singh AK, Singh, R. TRIM13, a novel regulator of Endoplasmic Reticulum Associated Protein Degradation pathway. XXXIV All India Cell Biology Conference and Symposium on “Quantitative Biology: from Molecules to Cells” Bose Institute Kolkata, West Bengal, India, December 04-06, 2010 (Oral presentation).

4. Tomar D, Singh R, Sripada L, Prajapati P, Singh AK, Singh R. TRIM8, a novel RING E3 ligase regulates NF-κB pathway and clonogenic/migration ability of the cells. International Symposium on Cancer Biology, National Institute of Immunology, New Delhi, India, November 14-16, 2011. Best poster award.

5. Sripada L, Tomar D, Prajapati P, Singh R, Singh AK, Singh R. Mitochondria: Preferred site of mature miRNA. SMRM 1st Annual Conference on "Mitochondria in Biology and Medicine, Centre for Cellular and Molecular Biology, Hyderabad, Andhra Pradesh, India, December 9-10, 2011.

6. Tomar D, Singh R, Singh AK, Singh R. TRIM13 regulates autophagosome biogenesis on ER membrane and suppresses clonogenic ability of cells via autophagy. The XXXV All India Cell Biology conference and symposium on Membrane dynamics and disease, National Institute of Science Education and Research (NISER), Bhubaneswar, Orissa, India, December 16-18, 2011.

7. Jain M, Prajapati P, Tomar D, Sripada L, Singh R, Singh AK, Singh R. TNF induces cell death in autophagy dependent manner in cell line model of Parkinson Diseases. 1st International Meet On Advance Studies in Cell Signaling Network, CSIR- Indian Institute of Chemical Biology, Kolkata, India, September 11-13, 2012.

8. Tomar D, Sripada L, Prajapati P, Singh R, Singh AK, Singh R. Nucleo-cytoplasmic translocation of TRIM8 is essential for positive regulation of TNF induced NF-κB pathway. 1st International Meet On Advance Studies in Cell Signaling Network, CSIR- Indian Institute of Chemical Biology, Kolkata, India, September 11-13, 2012 (Oral presentation).

9. Bhatelia K, Singh K, Tomar D, Lakshmi S, Prajapati P, Singh R. MITA induces cell death by regulation of NFB in MCF-7 breast cancer cell line. XXXVI All India Cell Biology Conference & International Symposium On “Stress Adaptive Response and Genome Integrity”, Bhabha Atomic Research Centre, Mumbai, India, October 17-19, 2012.