Dr. Budhi Sagar Tiwari, Ph.D, Associate Professor

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 Education

  • Ph.D. (Botany): Banaras Hindu University, Varanasi, India.
                

Research Interest


  •  Identifying Chloroplastic components involved in the execution of abiotic stress induced PCD:

In animals as well as plants mitochondria play a pivotal role in the regulation of upstream event of PCD induced by abiotic stress like oxidative, heat, salt and chemically induced PCD. Almost all abiotic stresses converge into oxidative condition and they lead to oxidative burst.  Mitochondria amplify ROS production through uncoupled e- transport chain leading to the depletion of cellular ATP content, opening of MTP (mitochondrial transition pore) and migration of cytochrome c from mitochondria to cytosol. In Planta, chloroplast represents another source for producing ROS through photochemical reaction of photosynthesis and through chlorophyll biosynthetic pathway. Although frequency and amount of ROS generation by chloroplast is almost equal or even higher than mitochondrial ROS generation, role of chloroplast in abiotic stress induced PCD is poorly known, some scattered and recent studies suggest that chloroplast might play a crucial role in the regulation of PCD. To go in to the deep, we are trying to identify regulatory components of chloroplastic residence that are involved in abiotic stress induced PCD. To decipher these components we are applying the tools of molecular biology and biochemistry.

  • Identifying Regulatory components underlying anhydrobiosis for their utility in dryland farming:

Drought is one of the major environmental cues severely affecting yield of the crops at the global level. A frequent and prolong drought can affect primarily soil health. Poor soil health doesn’t allow crops to withstand and complete life cycle. Under stressful conditions, crops show premature senescence which reduce yield in unmanageable amount. To meet the demand of food, it is utmost important to develop a strategy wherein crop can sustain environmental hardship without affecting it’s yield. There are piles of information available on molecular mechanisms involved in drought tolerance and there are several reports indicating utilisation key regulatory elements involved in drought tolerance to raise transgenic plants showing improvement in tolerance against water stress. In the most of the reports, candidate genes were identified from a species which has better tolerance capacity against drought.Interestingly, there are certain organisms which survive removal of almost all of their cellular water without irreversible damage, such organisms are referred as desiccation tolerant or anhydrobiotes. In contrast to drought tolerance where an organism survives a moderate amount of water loss, anhydrobiotes have ability to survive even after removing almost all of their cellular water without irreversible damage. In other words, when a completely dehydrated anhydrobiote is rehydrated with ample amount of water, it can resume life again. It indicates that anhydrobiotes have developed powerful mechanism to protect their macromolecules from desiccation induced damage. Therefore targeting key regulators of desiccation tolerance could be of utmost importance in crop improvement against drought stress.

Based on the concept mentioned above, in a long run I would like to keep myself involved in identifying key regulators of anhydrobiosis using tools of genomics and proteomics in combination (Proteo-genomics) in order to pin-point key regulators which can be tested in crop plants against drought.


Publications


1. Vivek Ambastha,  S.K. Sopory, B.C. Tripathy and Budhi Sagar Tiwari (2016) :  Photo-modulation of Chloroplast-Induced Programmmed Cell Death in Rice Leaves Triggered by Salinity. (Accepted for publication in Apoptosis)

2. Thagela P, Yadav RK, Mishra V, Dahuja A, Ahmad A, Singh PK, Tiwari BS, Abraham G. (2016) Salinity-induced inhibition of growth in the aquatic pteridophyte Azolla microphylla primarily involves inhibition of photosynthetic components and signaling molecules as revealed by proteome analysis. Protoplasma  [Epub ahead of print] Pubmed

3. Manas Kumar Tripathy, Budhi Sagar Tiwari, Renu Deswal and SK Sopory (2015) Ectopic Expression of PgRab7 influenced growth and enhanced tolerance to salinity and draught stress in Oryza sativa vc Indica. Protoplasma  [Epub ahead of print]

4. Vivek Ambastha, Baishnab C. Tripathy and Budhi Sagar Tiwari (2015) Programmed cell death in plants: a chloroplastic connection. Plant Signalling and Behavior 10(2). Pubmed

5. Jens F. Sundström, Alena Vaculova, Andrei P. Smertenko, Eugene I. Savenkov, Anna Golovko, Elena Minina, Budhi S. Tiwari, Salvador Rodriguez-Nieto, Andrey A. Zamyatnin Jr, Tuuli Välineva, Juha Saarikettu, Mikko J. Frilander,  Maria F Suarez, Anton Zavialov, Ulf Ståhl, Patrick J. Hussey, Olli Silvennoinen, Eva Sundberg, Boris Zhivotovsky and Peter V. Bozhkov. (2009) Tudor−SN an evolutionary conserved component of programmed cell death degradome. Nature:Cell Biology  11 (11) 1347-1354. Pubmed

6. Govrin E.M., Rachmilevitch, S., Tiwari, BS., Solomon, M., Belenghi, B. Levine, A (2006): A elicitor from Botrytis cinera induced hypersensitive response in Arabidopsis thaliana and other plants and promotes the gray mold disease. Phytopathology 96 (3) 299-307. Pubmed

7. Ricardo Capone, Tiwari, BS. & Alex Levine (2004): Rapid Transmission of Oxidative Stress Signal from Roots to Shoots in Arabidopsis thaliana. Plant Physiology & Biochemistry, 42 (5): 425-428 Pubmed

8. Alexander Mazel, Tiwari BS. and Alex Levine (2004): Induction of salt and osmotic stress tolerance by overexpression of an intracellular vesicle trafficking protein AtRab7 (AtRabG3e). Plant Physiology. 134(1),118-128 Pubmed

9. Tiwari, B S., Beatrice Belenghi and Alex Levine (2002) Oxidative stress increased respiration and generation of reactive oxygen species, resulting in ATP depletion, opening of mitochondrial permeability transition, and programmed cell death. Plant Physiology. 128 (4), 1261-1271 Pubmed

10. Chattopadhyay MK., Tiwari, BS. Chattopadhyay, G, Bose, A., Sengupta, DN.  & Ghosh, B. (2002) Molecular mechanism of polyamine action in salt tolerant and salt sensitive rice cultivars. Physiol Plant 116 (2): 192-199  

Contacts


Plant Cell & Molecular Biology
University & Institute of Advanced Research
Koba Institutional Area, Gandhinagar-382007, Gujarat, India

Emailbstiwari@iar.ac.in, budhi@rediffmail.com
Office Phone: +91-(0)79-30514145

Website: http://www.iiar.res.in/?q=node/467