Dr. Anand Krishna Tiwari
School of Biological Sciences and Biotechnology
Indian Institute of Advanced Research
Koba, Gandhinagar, 382 007
Ph.D.: 2008, Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi, India.
Post-doc: 2008-2010, Embryotoxicology Division, Indian Institute of Toxicology Research, Lucknow, India.
Drosophila Developmental Biology
Drosophila eye development:
Fly eye is a highly ordered structure made of ~750-800 units (ommatidia). An ommatidium consists of 20 cells that include eight photoreceptor, four cone, two primary, six secondary, three tertiary pigment cells and one bristle cells. Due to its precisely organized architecture, the fly eye is an effective model for the study of intracellular signaling, pattern formation, cell shape change/cell adhesion, polarity development and identification of genes related with several diseases. Molecular chaperones play a key role in protein quality control and are responsible for proper protein folding, transport across membrane, refolding of proteins denatured by environmental stress and intracellular transport. Hsp70, one of the well studied molecular chaperone bind to an unfolded protein substrate, and stabilizing its exposed hydrophobic regions. In Drosophila it has been demonstrated that Hsp70 acts as a potent suppressor for polyglutamine disease and Parkinsonâ€™s disease. When Hsp70 gene function was inhibited in fly eyes using a dominant negative form of Hsp70 an enhanced retinal degeneration/reduction in eye size/bristle abnormality was observed. Since the molecular chaperon Hsp70 is associated with proper protein folding as well as protein trafficking we are interested to understand the role of Hsp70 in eye development and rhodopsin (a major eye protein in Drosophila) folding and its trafficking to the rhabdomere base.
Drosophila as a model organism to study human neurodegenerative diseases:
Neurodegenerative diseases are group of disorders that include brain, peripheral nerves, spinal cord and leads to either functional loss or sensory dysfunction have been emerged as challenging problem in recent years and need immediate concern. Drosophila, the closest invertebrate model organism with ~75% similarity with known human disease genes has been established as a powerful animal model to understand the pathways of a number of Human diseases in order to find out the pharmacological targets. We are interested to use Drosophila as a model system to study the role of ubiquitin protein ligases and Hsp70 molecular chaperon in the pathogenesis of neurodegenerative diseases.
- Alone DP, Tiwari AK, Mandal L, Li M, Mechler BM, Roy JK (2005). Rab11 is required during Drosophila eye development. International Journal of Developmental Biology, 49: 873-879.
- Tiwari AK, Alone DP and Roy JK (2008). Rab11 is essential for fertility in Drosophila. Cell Biology International, 32: 1158-1168.
- Tiwari AK and Roy JK. (2009). Mutation in Rab11 results in abnormal organization of ommatidial cells and JNK activation in Drosophila eye. European Journal of Cell Biology, 88:445-460 .
- Guruprasad, BR, Tiwari AK, Hegde SN. (2010). Studies on the body melanisation of D. malerkotliana of Mysore. Dros. Inf. Serv. 200-201.
- Pant DC, Dave M, Tiwari AK. (2013). Wheatgrass (Triticum aestivum L.) supplementation promotes longevity in Drosophila melanogaster. Annals of Plant Sciences, 49-54.
- Kumar A, Dave M, Pant DC, Luxar R, Tiwari AK. (2013). Vinca rosea leaf extract supplementation leads to developmental delay and several phenotypic anomalies in Drosophila melanogaster. Toxicological & Environmental Chemistry, 95:635-645.
- Tiwari AK. (2013). Drosophila melanogaster: A Genetics and Biological Research Model. India Education Newsletter. October, 5-8.
- Tiwari AK, Panda P, Purohit JS. (2014). Evaluation of sub-cellular distribution of glutamate dehydrogenase (GDH) in Drosophila melanogaster larvae. Acta Histochemia.116: 297-303.
- Shukla AK, Pargya P,Singh HC, Tiwari AK, Patel D K, Abdin MZ and Chowdhuri DK (2014). Heat shock protein-70 (HSP70) suppresses paraquat-induced neurodegeneration in Drosophila model of Parkinson`s disease. Plos One, 9:1-11.
- Sonani R, Rastogi R, Singh NK, Thadani J, Patel PJ, Kumar J, Tiwari AK,
Devkar RV, Madamwar D (2016). Phycoerythrin averts intracellular ROS generation and physiological functional decline in eukaryotes under oxidative stress. Protoplasma. DOI 0.1007/s00709-016-0996-5.
Panchal K, Patel K, Tiwari AK (2016). Dietary Supplementation of Citric acid (monohydrate) Improves Health Span in Drosophila melanogaster. Journal of Applied Biology & Biotechnology Vol. 4 (02), pp. 060-066.
- Tiwari AK, Prakash P, Ravi RK, Chowdhuri DK (2011). Environmental chemical mediated male reproductive toxicity: Drosophila melanogaster as an alternate model organism, Theriogenology, 76:197-216.
- Guruprasad BR, Kumar S, Tiwari AK. (2010). Designing a potential Inhibitor for Tyrosin Kinase. ISBN No. 978-3-8383-9248-6, LAP LAMBERT Academic Publishing.
- Guruprasad BR, & Tiwari AK. (2011). Handbook of insect Biology. Discovery Publishing House Pvt Ltd, ISBN No. 8183569102.
- Prof. B. R. Sheshachar award for best paper presentation in XXIX All India Cell Biology Conference & Symposium on Gene to Genome organized by Industrial toxicological Research Centre (ITRC), Lucknow, 2006.
2. Best Poster Award in “National Conference on New Frontiers in Medicinal Plant Research” at Sikkim University, Sikkim, 2013.
Member of scientific society:
- Life member, Indian Society of Cell Biology, India.
- Member of Indian Society of Developmental Biologist India
Award winning confocal images: